[10p-N305-1~12] 12.7 Biomedical Engineering and Biochips

2:30 PM - 2:45 PM JST(5:30 AM - 5:45 AM UTC)

[10p-N305-5] Migration Analysis of Cardiac Fibroblasts on One-Dimensional grooved PDMS Substrates

〇Hiromu Kuwabara¹, Aoki Kazuhiro¹, Yuuta Moriyama¹, Toshiyuki Mitsui¹ (1.Aogaku Univ.)

Keywords:

Dynamics,Three-dimensional culture,PDMS

We fabricated one-dimensional (1D) microgroove structures on PDMS substrates with groove widths of 30-100 µm, heights of 45 µm, and inter-groove spacings of 50-150 µm. Chick embryonic heart-derived cardiomyocytes and fibroblasts were locally co-cultured on these substrates. When these cells were allowed to migrate freely along the 1D microgrooves, they exhibited distinct motilities. Notably, the condition with 80 µm inter-groove spacing resulted in the fastest transition to confluency. This study aims to control the directed migration of fibroblasts within cardiac tissue using 1D microgroove structures.