[8a-P06-1~10] 12.6 Nanobiotechnology

[8a-P06-1] Detection Angle Dependence of Luciferin Chemiluminescence
on the Plasmonic Chip

〇Shunta Nakatsuji¹, Hitomi Yamanaka¹, Riku Shimosaka¹, Yasunori Nawa¹, Keiko Tawa¹ (1.Kwansei Gakuin Univ.)

Keywords:

chemiluminescence,biosensor,detection angle

An excited state of oxyluciferin is formed from luciferin in the presence of adenosine triphosphate (ATP), O₂, Mg⁺, and the enzyme luciferase, and chemiluminescence is detected in the transition process to the ground state. So far, the luminescence enhancement has been observed at a pitch (Λ) of 480 nm in a plasmonic chip with a silver-coated periodic structure compared to a flat silver substrate. In this study, we investigated the plasmon enhancement of luciferin luminescence and the detection angle dependence of luminescence.