[15a-P04-1~13] 12.6 Nanobiotechnology

[15a-P04-4] Rapid, Bisulfite-free, and Label-free Detection of DNA Methylation Based on Photonic Crystal Biosensor

〇(B)Kota Yamamoto¹, Hideaki Hisamoto¹, Tatsuro Endo¹ (1.Osaka Metropolitan Univ.)

Keywords:

Photonic crystal,Biosensor,Epigenome

DNA methylation detection technologies are expected to contribute to the early diagnosis of cancer, lifestyle-related diseases, and mental illnesses. In this study, using a photonic crystal biosensor with periodic nanostructures, DNA hybridization detection and subsequent DNA methylation detection via an anti-methylated DNA antibody reaction were achieved within a total of 2 hours, without the need for staining or bisulfite treatment.