[16a-K402-1~8] CS.7 Code-sharing Session of 12.6 & 12.7

9:15 AM - 9:30 AM JST(12:15 AM - 12:30 AM UTC)

[16a-K402-2] Secondary Structure Analysis of Proteins Immobilized on Amino-EG6-Undecanethiol Monolayers Using In Situ Surface-Enhanced Infrared Absorption Spectroscopy with Gold Nanostructures

〇(PC)Hiroyuki Yamada¹, Thien Duc NGO¹, Tadaaki Nagao^1,2 (1.NIMS, 2.Hokkaido Univ.)

Keywords:

surface enhanced infrared absorption spectroscopy,lysozyme,in situ observation

In this study, an amino-EG6-undecanethiol self-assembled monolayer (SAM) was formed on a gold random nanostructure surface fabricated in PBS aqueous solution, and the secondary structure of lysozyme immobilized on this SAM was analyzed using in situ surface-enhanced infrared absorption spectroscopy (SEIRA). The results revealed differences in the shape of the infrared absorption spectrum in the amide I region compared to the bulk, suggesting changes in the secondary structure.