Presentation Information
[17p-K201-5]Phosphate transport and response mechanisms revealed by micro-regional
beta ray imaging.
〇Satomi Kanno1 (1.Nagoya Univ. IAR)
Keywords:
Scintillator,Beta ray,Plants
Understanding where, when and how much of nutrient elements are present in vivo is necessary for studying transport mechanisms. To understand this, we collect and fix plants for analysis, which is not easy when we want to carry out continuous analysis on the same experimental sample. Furthermore, it is not easy when the analysis is performed on microtissues with low amounts of target elements. This study presents the development of methods for analyzing classical radioisotope tracers with high spatio-temporal resolution using lenses and the latest image sensors. These systems have been used to analyze the in vivo behavior of phosphate tracer as a model. We analyzed the behavior of phosphate tracers from the whole plant level to the intercellular level and revealed differences in their accumulation. Phosphate tracer imaging of transgenic lines was used to characterize the function of phosphate transporters in vivo and to determine the high uptake contribution of root tip cells to the whole plant. The heterogeneous accumulation of tracers also leads to an understanding of the differences in response to phosphate between tissues.
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