Presentation Information
[15a-WL1_201-10]Suppression of nonspecific adsorption on plasmonic chip modified with photoresponsive molecules
〇Moe Taniguchi1, Kyohei Asano1, Yasunori Nawa1, Mutsuo Tanaka2, Keiko Tawa1 (1.Kwansei Gakuin Univ., 2.Saitama Inst. of Tech.)
Keywords:
plasmon,photo chemical reaction,multi-array biosensor
The plasmonic chip is a substrate with a wavelength-size periodic structure covered with thin metal layers. In our laboratory, the electric field enhanced with the Bull’s eye-type plasmonic chip was applied to not only the fluorescence enhancement but also the promotion of photochemical reaction. The chip modified to photo-responsive molecule (4-((2-folmyl-3-methylphenyl)thio) benzoic acid) (O-MBA) was irradiated with visible light, and intermediates of B-O-MBA were formed in the exposed area. On the structure of plasmonic chip, this photochemical reaction is promoted and maleimide compounds are densely bound. We aim to construct multi-array type sensitive biosensors with plasmonic chips. In this study, we evaluated fluorescence intensity on a substrate bound via biotin-avidin interaction using biotin-maleimide (Biotin-M) and Cy5-labeled streptavidin (Cy5-SA), with suppression of non-specific adsorption.