[15a-WL1_301-1~11] 12.7 Biomedical Engineering and Biochips

11:30 AM - 11:45 AM JST(2:30 AM - 2:45 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[15a-WL1_301-10] Investigation of Molecularly Imprinted Polymer Film Formation Conditions on an Optical Interferometric MEMS Sensor for Highly Sensitive Dopamine Detection

〇Ikuto Tazawa¹, Masaki Oshino¹, Junki Hirano¹, Ik Hyun Kwon¹, Yong-Joon Choi¹, Toshihiko Noda¹, Kazuaki Sawada¹, Kazuhiro Takahashi¹, Hiroyuki Ohta² (1.Toyohashi Univ. Tech., 2.NDMC)

Keywords:

Molecularly Imprinted,Micro Electro Mechanical Systems,biomarker

Accurate disease diagnosis requires the detection of biomarkers that reflect disease progression. In coronavirus disease, cytokine concentrations change at early symptoms, while catecholamines concentrations change at degree of severity, necessitating simultaneous detection of high- and low molecular-weight biomolecules. While high-molecular-weight biomarkers can be detected down to 100 ag/mL (PSA) using antigen–antibody reactions, low-molecular-weight detection remains challenging. Therefore, molecularly imprinted polymer (MIP) films have been employed as artificial receptors for dopamine. To improve the detection sensitivity of MIP sensors, the dopamine-to-pyrrole ratio during electropolymerization was optimized. Excess dopamine caused freestanding membrane instability, and the optimal ratio was determined to be 1:4. Under this condition, surface stress responses approximately three times larger than those in previous studies were obtained, demonstrating improved detection sensitivity for low-molecular-weight biomolecules.