[15p-M_107-1~15] 8.4 Plasma life sciences

4:30 PM - 4:45 PM JST(7:30 AM - 7:45 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[15p-M_107-10] Changes in antioxidant gene expression in MCF-7 cells after repeated treatment with plasma-activated Ringer’s lactate solution

〇Ryo Wakatsukasa¹, Ayako Tanaka², Camelia Miron², Kae Nakamura^2,3, Kenji Ishikawa^1,2, Masaru Hori², Hiromasa Tanaka^1,2 (1.Nagoya Univ. Eng., 2.Nagoya Univ. Center for Low-temperature Plasma Sciences, 3.Nagoya Univ. Med.)

Keywords:

plasma medicine,oxidative stress,chemoresistance

In our previous study, we reported that P-MCF-7 cells, which were generated by repeated treatment of human breast cancer cells (MCF-7) with plasma-activated lactated Ringer’s solution (PAL), exhibited reduced sensitivity to PAL, and that this reduced sensitivity was attributable to decreased sensitivity to hydrogen peroxide (H₂O₂). In the present study, to elucidate the underlying mechanism, we analyzed the expression levels of antioxidant genes in P-MCF-7 cells. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression levels of catalase (CAT) and superoxide dismutase 2 (SOD2) were lower in P-MCF-7 cells than in parental MCF-7 cells. These results suggest that the reduced H₂O₂ sensitivity in P-MCF-7 cells may be mediated by the upregulation of other antioxidant genes or by mechanisms independent of antioxidant activity.