[15p-M_107-1~15] 8.4 Plasma life sciences

4:00 PM - 4:15 PM JST(7:00 AM - 7:15 AM UTC)

[15p-M_107-8] Apoptosis Induction in Spheroid-Cultured HSC-3 Oral Cancer Cells by Atmospheric Pressure Plasma Irradiation

Eikichi Morita¹, 〇Yoshihito Yagyu¹, Nobuya Hayashi¹ (1.Kyushu Univ.)

Keywords:

three-dimensionally cultured cells,Spheroid-Cultured,Cold atmospheric plasma

The development of cancer treatments with high target selectivity and minimal invasiveness remains a critical challenge. Cold atmospheric plasma (CAP) has attracted attention as a novel cancer therapy because it generates various reactive oxygen and nitrogen species under low-temperature conditions and demonstrates antitumor effects. However, conventional two-dimensional monolayer cultures do not adequately reproduce the tumor microenvironment, limiting accurate evaluation of CAP efficacy and mechanisms. In this study, we evaluated the effects of CAP irradiation on HSC-3 human oral cancer cells using a three-dimensional spheroid model. In particular, apoptosis induction in three-dimensionally cultured cells was investigated.