[15p-M_110-1~12] 3.3 Biomedical optics

2:30 PM - 2:45 PM JST(5:30 AM - 5:45 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[15p-M_110-3] Real-time Mid-infrared Photothermal Hyperspectral Volumetric Imaging

〇(D)Masato Fukushima¹, Keiichiro Toda¹, Yusei Sugawara¹, Shotaro Kawano¹, Takuro Ideguchi¹ (1.The Univ. of Tokyo)

Keywords:

mid-infrared spectroscopy,hyperspectral imaging,tomography

Mid-infrared photothermal (MIP) microscopy can provide chemical contrast at visible-light spatial resolution; however, three-dimensional, real-time spectroscopic imaging has not yet been established. In this study, we integrated MIP microscopy with optical diffraction tomography (ODT), which enables 3D reconstruction from a limited number of illumination angles, and realized a 3D MIP-ODT microscope operating at 19.2 Hz. Furthermore, by rapidly sweeping the mid-infrared range of 2800–3100 cm^-1 at 300 cm^-1/s and acquiring 20 spectral points per second, we distinguished spectral differences between lipid droplets and the nucleolus in COS-7 cells.