[15p-M_110-1~12] 3.3 Biomedical optics

3:30 PM - 3:45 PM JST(6:30 AM - 6:45 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[15p-M_110-6] High-speed multi-color imaging by the integration of line-illumination Raman microscopy and bright and low-fluorescence Raman tag nanoparticles

〇Ikuto Eshima¹, Ryo Harada¹, Ryo Sakamoto¹, Yuki Kitayama¹, Soma Yumoto¹, Ryosuke Oketani¹, Hiroyoshi Fujioka², Mako Kamiya², Kotaro Hiramatsu¹ (1.Kyushu Univ., 2.Science Tokyo.)

Keywords:

Raman imaging,Raman tag,Multiplex imaging

Raman tag imaging is a multicolor imaging method that enables identification of multiple labeled molecules; however, its temporal resolution is limited by the low signal intensity of spontaneous Raman scattering. In this study, we demonstrate a high-speed and broadband Raman tag imaging platform by integrating a line-illumination Raman microscopy with bright and low-fluorescence Raman tag nanoparticles. As a proof of principle, live-cell Raman imaging was performed, and the distributions of exogenous tags and endogenous molecules were visualized within 52 s over a field of view of 512 pixels × 100 lines.