Presentation Information
[15p-M_110-8]Contrast enhancement in time-resolved stimulated Raman scattering microscopy using multi-pulse selective excitation
〇(B)Tsubasa Miyaoka1, Terumasa Ito1 (1.Tokyo Univ. of Agriculture and Technology)
Keywords:
Bioimaging,Raman microscopy,Stimulated Raman Scattering
We developed time-resolved SRS microscopy using multi-pulse selective excitation to detect low-concentration drugs in biological tissues with high contrast. This method utilizes phase modulation of ultrashort pulses to suppress the excitation of background Raman signals while selectively exciting the target signal. Experimental results confirmed an improvement in contrast compared to conventional methods, enabling clear visualization of the spatial concentration distribution of the drug. These findings demonstrate that this approach is effective in addressing the challenge of suppressing background molecular signals in biological measurements.