[15p-M_278-1~8] Cutting edge nanotechnology for bio-sensor & 2D materials -Realization of a pandemic-free society with graphene FET sensors capable of rapid detection of human infectious viruses-

3:45 PM - 4:00 PM JST(6:45 AM - 7:00 AM UTC)

[15p-M_278-6] Membrane Protein Analysis Using a Plasmonic Chip of Single Extracellular Vesicles Secreted from Cultured Cells

〇Yasunori Nawa¹, Tatsuki Iwamura¹, Makoto Tokami¹, Hajime Shigeto², Shohei Yamamura², Keiko Tawa¹ (1.Kwansei Gakuin Univ., 2.AIST)

Keywords:

Exosome,Enhanced fluorescence,Plasmonic chip

Exosomes are extracellular vesicles (EVs) with diameters of approximately 100 nm that mediate intercellular communication by transferring proteins and nucleic acids secreted from cells. Because of their sub-micrometer size and weak optical signals, optical detection and analysis of single EVs remain challenging. In this study, we developed a fluorescence-enhancement technique using a plasmonic chip with concentric periodic nanostructures covered with a metal layer. Single EVs were detected as fluorescent spots via a sandwich immunoassay using upright-inverted fluorescence microscope. By employing different capture antibodies, membrane proteins on individual EVs were selectively analyzed. EVs derived from normal cells and MCF-7 breast cancer cells were successfully detected and quantified, while negligible signals were observed in control samples. Furthermore, EV release from MCF-7 cells exhibited a dependence on the culture duration.