[16p-W2_402-1~17] 12.6 Nanobiotechnology

3:45 PM - 4:00 PM JST(6:45 AM - 7:00 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[16p-W2_402-9] Ultrasensitive Single-Molecule Quantum Detection of O⁶-Methylguanine for Brain Tumor Diagnostics

〇Shinya Sakano¹, Takahito Ohshiro², Yuki Komoto², Masateru Taniguchi² (1.Susumu CO., LTD., 2.Sanken, Osaka Univ.)

Keywords:

single-molecule detection,Tunnel-Current,Biopolymer

DNA and other nucleic acids undergo various chemical lesions through alkylation and oxidation, contributing to cell death and genomic instability. Among these, O⁶-methylguanine (O⁶-MeG), induced by the brain tumor drug temozolomide (TMZ), is a key determinant of drug sensitivity, together with its repair by MGMT (O⁶-methylguanine-DNA methyltransferase). However, because such lesions occur at extremely low frequency and coexist with oxidative damage (e.g., 8-oxoG), conventional ensemble measurements cannot directly quantify O⁶-MeG. Here, we present a single-molecule quantum sensing approach using nanogap electrodes to discriminate O⁶-MeG, 8-oxoG, and normal nucleic acids based on their electrical fingerprints and dynamic behavior. This technique, previously applied to nucleic acids and amino acids, enables high-precision analysis of damaged nucleic acids, offering new insights for brain tumor diagnostics and drug response evaluation.