[16p-WL1_201-1~14] Innovation in Physical Chemistry and Quantum Life Sciences via Photothermal Conversion

3:25 PM - 3:40 PM JST(6:25 AM - 6:40 AM UTC)

[16p-WL1_201-6] Toward Exploring Quantum Effects in DNA–DNA Interactions under Hetero-Probe Optical Condensation

〇Shuichi Toyouchi¹, Syoji Ito^1,2, Mamoru Tamura^1,3, Kota Hayashi^1,4,5, Yumiko Takagi¹, Ayumu Taguchi^1,6, Shiho Tokonami^1,5, Takuya Iida^1,4 (1.RILACS in Osaka Met. Univ., 2.Grad. Sch. Eng. Sci. in Osaka Univ., 3.Grad. Sch. Sci. in Kwansei Gakuin Univ., 4.Grad. Sch. Sci. in Osaka Met. Univ., 5.Grad. Sch. Eng. in Osaka Met. Univ., 6.Grad. Sch. Med. in Nagoya City Univ.)

Keywords:

Optical condensation,DNA,Photothermal effects

We investigated the positional dependence of single-base mismatch using a hetero-probe optical condensation method developed for highly sensitive DNA detection. In this method, target DNA is selectively condensed via duplex formation between DNA-modified microbeads and gold nanoparticles under laser irradiation. We found that when a mismatch base is introduced at positions close to the gold nanoparticle, the sequence specificity is significantly reduced, and this reduction strongly depends on the laser intensity used for optical condensation. These results suggest that the local environment near the gold nanoparticle plays a critical role in modulating DNA duplex formation. Possible contributions from photothermal conversion–induced local temperature rise, hot-electron generation, electron injection into DNA adsorbed on the gold nanoparticle surface, and proton transfer mediated by quantum tunneling between base pairs are considered to discuss the local modulation mechanism of DNA duplex formation.