Presentation Information
[17a-PB3-3]Development of Cell-Type-Specific Microelectrodes for Measuring Neural Activity
Jinwoo Kim1, Mieko Imayasu1, Tomoyuk Yoshida2, 〇Hidekazu Tsutsui1 (1.JAIST, 2.Toyama Univ)
Keywords:
neuron,microelectrode,synapse organizer
Conventional microelectrode for recording neuronal activity lacks inherent cell-type selectivity, which is one of the major limitations for detailed analysis of neuronal circuits. In this study, we engineered orthogonal molecular tools of compact artificial synapse organizers based on neurexin1β and peptide-tags, fabricated gold microelectrodes functionalized with the corresponding receptor, and conducted validation experiments using primary cultured neurons in-vitro. Synapse-like junctions were successfully induced at contact sites between neurons expressing the engineered synapse organizer and the functionalized microelectrodes, but not in the negative control experiments without electrode functionalization. We’d expect that this molecularly inducible neuron–microelectrode junction may serve as a basis for next-generation microelectrode techniques that enable cell-type-selective recording.