Presentation Information
[17a-PB3-4]Direct observation of DNA translocation and clogging in PEG Solutions: orientation-dependent effects in slit-shaped Nanopores
〇Naoki Kato1, Takuma Yoshinaga1, Yugo Hatano1, Yuuta Moriyama1, Toshiyuki Mitsui1 (1.Aoyama Univ)
Keywords:
DNA,nanopore,thin film
The behavior of DNA entry and clogging in solid-state nanopores was directly observed in highly viscous aqueous solutions of polyethylene glycol (PEG). Slit-shaped nanopores with aspect ratios of 1:1, 1:5, and 1:20 exhibited clear orientation-dependent anisotropy in DNA entry dynamics. The addition of high–molecular-weight PEG (PEG20000) significantly reduced the clogging probability compared with PEG-free solutions. In particular, DNA entering perpendicular to the long axis of the slit-shaped nanopore showed a markedly reduced clogging probability down to 24%. These results demonstrate that both solution viscosity and pore geometry play critical roles in possible elimination of DNA clogging in nanopore-based biosensing.