[17a-WL1_301-1~11] 12.7 Biomedical Engineering and Biochips

9:15 AM - 9:30 AM JST(12:15 AM - 12:30 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[17a-WL1_301-2] Development of a Rapid General Viable Bacterial Counting Method Based on Current Response of Tetrazolium Salts

〇(D)Hikaru Ikeda¹, Kanta Sako¹, Satohiro Itagaki¹, Masashi Fujita², Hiroshi Shiigi¹ (1.Osaka Metro. Univ., 2.EC FRONTIER)

Keywords:

MTT assay,General Viable Bacteria,electron mediator

After the tetrazolium salt MTT passes through the cell membrane, it is intracellularly reduced by endogenous electron carriers, such as reduced coenzymes, to insoluble formazan, which accumulates inside the cells. Immediately after dissolving MTT in a bacterial suspension, a distinct current response attributable to MTT was observed; however, the current response decreased after 1 h of incubation. This decrease is attributed to the uptake of MTT into bacterial cells and the consequent reduction in the concentration of residual MTT remaining in the suspension. In this study, based on electrochemical measurements of the remaining MTT, we successfully developed a method for measuring general viable bacterial counts within 1 h.