[2P] Intracellular Transport, Organelles, and Biological Membranes

1:10 PM - 2:50 PM JST(4:10 AM - 5:50 AM UTC)

[2P48] Optimized Isolation of Glioblastoma Nuclei for High-Speed AFM: Real-Time Visualization of Nuclear Pore Complex (NPC) Dynamics

*Xin Zhang¹, Mahmoud Shaaban Mohamed³, Keesiang Lim², Yujia Qiu¹, Richard W Wong^1,2,3 (1. Division of Nano Life Science, Graduate School of Frontier Science Initiative, Kanazawa University, Kanazawa 920-1192, Japan., 2. WPI-Nano Life Science Institute, Kanazawa University, Kanazawa 920-1192, Japan., 3. Cell-Bionomics Research Unit, Institute for Frontier Science Initiative, Kanazawa University, Kanazawa 920-1192, Japan.)

Keywords:

nuclear pore complexes (NPCs),high-speed atomic force microscopy (HS-AFM),U87 cell line,nuclear isolation,Nucleus-Enriched Filtration Technique (NEFT)

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