The 48th Annual Meeting of the Molecular Biology Society of Japan
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Session Details

Symposium

[1PS-04]【J】The new trend in knock-in technology that is expanding from CRISPR-Cas9

Wed. Dec 3, 2025 2:20 PM - 4:20 PM JST
Wed. Dec 3, 2025 5:20 AM - 7:20 AM UTC
Room 4(Pacifico Yokohama Conference Center 3F, 303)
Organizer: Masato Ohtsuka (Tokai University), Seiya Mizuno (University of Tsukuba)
Demand for gene knock-in technology, which accurately inserts a DNA fragment into a target gene locus, is extremely high, necessitating the development and improvement of methods that can perform this process simply and efficiently. This symposium will introduce a variety of approaches and related technologies for gene knock-in, from classical methods to the latest technologies. The speakers will discuss advantages, challenges and its application of the technology, as well as future prospects of each method.
2:20 PM - 2:24 PM JST(5:20 AM - 5:24 AM UTC)

Introduction

2:24 PM - 2:34 PM JST(5:24 AM - 5:34 AM UTC)

[1PS-04-01]Practical and Realistic Results of knock-in mice generation by zygote genome editing with CRISPR-Cas9.

○Natsuki Mikami1,2, Hayate Suzuki2, Yoko Tanimoto2, Kanako Kato2, Miyuki Ishida2, Natsumi Iki2, Hiromi Sakata2, Yoshikazu Hasegawa2, Daitoku Yoko2, Megumi Takemura2, Satoru Takahashi2, Seiya Mizuno2 (1. Ph.D Program in Human Biology, University of Tsukuba, 2. Laboratory of Animal Resource Center, University of Tsukuba)
Comment()
2:34 PM - 2:46 PM JST(5:34 AM - 5:46 AM UTC)

[1PS-04-02(1P-066)]Activation of non-canonical DSB repair pathways acceralate CRISPR-Cas9 mediated HDR

○Shogo Yamamoto1, Shoma Takashima2, Daisuke Motooka3, Daisuke Tachibana1, Junpei Yamamoto2, Shigenori Iwai2, Keiichiro Suzuki1,2,4 (1. Grad. Front. Bio., UOsaka, 2. Grad. Eng. Sci., UOsaka, 3. Res. Ins. Micro. Dis., UOsaka, 4. Ins. Ad. Co-cre. Stu., UOsaka)
Comment()
2:46 PM - 3:01 PM JST(5:46 AM - 6:01 AM UTC)

[1PS-04-03]Super Ultra Great Delicious Wonderful Knock-In in Pigs: The Cutting Edge of High-Efficiency Knock-In via In Vitro Fertilized Embryos

○Arata Honda1 (1. Jichi Medical University)
Comment()
3:01 PM - 3:13 PM JST(6:01 AM - 6:13 AM UTC)

[1PS-04-04(1P-016)]Development and optimization of gene knock-in technology with the elimination of a large genomic fragment using CRISPR-Cas3 in human cultured cells

○Daiki Nagatomo1,2,3, Yusuke Kojima3, Kazuto Yoshimi4, Tomoji Mashimo4, Akitsu Hotta3, Tetsushi Sakuma2 (1. Grad. Sch. Med., Kyoto Univ., 2. Grad. Sch. Agri., Kyoto Univ., 3. CiRA, Kyoto Univ., 4. Div. Animal Genet., Lab. Animal Res. Fac., Inst. of Med. Sci., Univ. of Tokyo)
Comment()
3:13 PM - 3:28 PM JST(6:13 AM - 6:28 AM UTC)

[1PS-04-05]Integrase-Mediated Targeted Transgenesis in Mice

○Hiromi Miura1, Ayaka Nakamura2, Aki Kurosaki1, Gurumurthy Channabasavaiah B.3, Masato Ohtsuka1 (1. Divi. of Bas. Med. Sci. and Mol. Med., Tokai Univ. Sch. of Med., 2. Life Sci. Support Cent., Tokai Univ., 3. Univ. of Nebraska Med. Cent.)
Comment()
3:28 PM - 3:46 PM JST(6:28 AM - 6:46 AM UTC)

[1PS-04-06]Programmable gene integration by the R2 retrotransposon coupled with CRISPR-Cas9

○Hiroshi Nishimasu1 (1. The University of Tokyo)
Comment()
3:46 PM - 4:01 PM JST(6:46 AM - 7:01 AM UTC)

[1PS-04-07]Strategy to generate tandem gene arrays on genomes with the gene duplication method.

○Hiroaki Takesue1, Satoshi Okada1, Takashi Ito1 (1. Dept. Biochem., Grad. Sch. Med. Sci., Kyushu Univ.)
Comment()
4:01 PM - 4:16 PM JST(7:01 AM - 7:16 AM UTC)

[1PS-04-08]A Practical Platform for Genome Integration of Designed Synthetic DNA

○Shunsuke Takahashi1 (1. Div. Life Sci. Eng., Sch. Sci. Eng., Tokyo Denki Univ.)
Comment()
4:16 PM - 4:20 PM JST(7:16 AM - 7:20 AM UTC)

Conclusion

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