Session Details
[1LS06]Immunohistochemistry Seminar for Neuroscience Researchers
- Key Points for Protocol Development and Evaluation to Improve Data Quality -
Thu. Jul 30, 2026 12:00 PM - 12:50 PM JST
Thu. Jul 30, 2026 3:00 AM - 3:50 AM UTC
Thu. Jul 30, 2026 3:00 AM - 3:50 AM UTC
Room 6 (501)
Moderator: Naoto Shimada (FUJIFILM Wako Pure Chemical Corporation)
Co-hosted by FUJIFILM Wako Pure Chemical Corporation
Immunohistochemistry is an exceptionally versatile and powerful technique; however, insufficiently validated signals and overinterpretation remain persistent concerns in the literature. This lecture highlights key strategies for reliable cellular and subcellular localization analysis: (1) stringent evaluation of primary antibodies; (2) rigorous use of negative controls, including knockout tissues; (3) optimization of fixation and staining protocols, with a particular focus on glyoxal-based fixation and antigen retrieval; and (4) consistency assessments through correlation with mRNA distribution and comparative analysis across brain regions. Through sharing specific protocols and practical troubleshooting insights, I hope to discuss the essential considerations for generating localization data that are reliable, reproducible, and convincing.
[1LS06-Introduction]Introduction
[1LS06-01]Revealing Previously “Invisible” Molecules: Fixation and Immunostaining Strategies for Advanced Tissue Localization
*Miwako Yamasaki1 (1. Department of Anatomy and Embryology, Department of Anatomy, Hokkaido University Graduate School of Medicine)
[1LS06-02]Pioneering the Future of Neuroscience Research - The Challenges and Potential of Fujifilm Wako's Antibodies -
*Shiro Sugino1 (1. FUJIFILM Wako Pure Chemical Corporation)