講演情報
[15a-WL1_301-5]Magnetic field-guided migration of corneal epithelial cells for accelerating wound healing
〇(M2)YUXIAO QIAN1 (1.Waseda University)
キーワード：
cell migration
Treatment of corneal injury depends on the severity of the injury and may employ methods such as artificial tears, antibiotics, therapeutic contact lenses, or surgical repair, aiming to promote epithelial healing, prevent infection and scarring, while restoring transparency and visual function. Beyond these conventional treatments, emerging regenerative strategies aim to actively control cell localization and migration to enhance tissue repair. Previous studies have explored the use of magnetic nanoparticles to label cells and guide their movement or positioning using external magnetic fields. For instance, Yanai et al. [1] used superparamagnetic iron oxide nanoparticles to lead stem cells to the retinal area for the treatment. Additionally, Tseng, P. et al. [2] demonstrated cell manipulation under high gradient magnetic fields using the force equation which standardized the movement patterns of magnetic nanoparticles in the cellular environment. Numerous studies on the effects of magnetic fields on cell proliferation, differentiation, apoptosis, etc., have been conducted, but their influence on directed cell migration has been discussed only to a limited extent.
This study investigated the effect of static magnetic fields on the migration of cells (HCE-T) without adding any magnetic nanoparticles, to explore the feasibility of accelerating wound healing. As shown in Fig.1, anti-Helmholtz coil system was built, and scratch assays of HCE-T were prepared and tested under four conditions: no magnetic field (control), magnetic center, left-side, and right-side gradient. The migration patterns were quantitatively analyzed by ImageJ, while COMSOL Multiphysics simulations were conducted to visualize the field distribution. Meanwhile, temperature tests were conducted to ensure that thermal conditions would not influence cell migration.
This study investigated the effect of static magnetic fields on the migration of cells (HCE-T) without adding any magnetic nanoparticles, to explore the feasibility of accelerating wound healing. As shown in Fig.1, anti-Helmholtz coil system was built, and scratch assays of HCE-T were prepared and tested under four conditions: no magnetic field (control), magnetic center, left-side, and right-side gradient. The migration patterns were quantitatively analyzed by ImageJ, while COMSOL Multiphysics simulations were conducted to visualize the field distribution. Meanwhile, temperature tests were conducted to ensure that thermal conditions would not influence cell migration.