The 105th CSJ Annual Meeting
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Academic Program [Oral A]17. Biofunctional Chemistry, Biotechnology : Oral A

[[A]A301-4am]17. Biofunctional Chemistry, Biotechnology

Sat. Mar 29, 2025 9:00 AM - 11:40 AM JST
Sat. Mar 29, 2025 12:00 AM - 2:40 AM UTC
[A]A301(A301, Bldg. 1, Area 3 [3F])
9:00 AM - 9:10 AM JST(12:00 AM - 12:10 AM UTC)Japanese

[[A]A301-4am-01]Association property of an antibody light chain that oligomerizes by 3D domain swapping and the effect of metal ions on the oligomerization

○Suzuno Takahashi1, Takahiro Sakai1, Shohei Yamaguchi1, Tsuyoshi Mashima1, Naoya Kobayashi1, Hideaki Ogata2, Emi Hifumi3, Taizo Uda4, Shun Hirota1 (1. Grad. Sch. Sci. Tech., NAIST, 2. Grad. Sch. Sci., Univ. Hyogo, 3. Inst. Res. Mgmt., Oita Univ, 4. Nano-tech Lab., ISIT)
9:10 AM - 9:20 AM JST(12:10 AM - 12:20 AM UTC)Japanese

[[A]A301-4am-02]Studies on association property of light chain variable region of therapeutic antibody rituximab

○Nambu Renri1, Sakai Takahiro1, Mashima Tsuyoshi1, Kobayashi Naoya1, Hifumi Emi2, Uda Taizo3, Hirota Shun1 (1. Grad. Sch. Sci. Tech., NAIST, 2. Inst. Res. Mgmt., Oita Univ, 3. Nano-tech Lab., ISIT)
9:20 AM - 9:30 AM JST(12:20 AM - 12:30 AM UTC)Japanese

[[A]A301-4am-03]Study on chemical modification of VHH antibodies (Ⅱ) : Reduction and HPLC analysis of anti-EGFR-VHH antibodies

○KENGO INAGAKI1, Takahiko Matsushita1,2,3, Tetsuo Koyama1, Ken Hatano1,2,3, Koji Matsuoka1,2,3 (1. Grad.Sci.&Engin., 2. Adv.Inst.Innov.Tech.(AIIT), 3. Health Sci., Strategic Res.Ctr)
9:30 AM - 9:40 AM JST(12:30 AM - 12:40 AM UTC)Japanese

[[A]A301-4am-04]N-terminal Fluorescent Labeling of Antibodies Using Triazolecarbaldehyde Derivatives and Evaluation of Antigen-Binding Affinity

○Yuya Maeda1, Takanobu Yasuda2, Tetsuya Kitaguchi2, Akira Onoda1,3 (1. Grad. Sch. of Env. Sci., Hokkaido Univ, 2. IIR, Science Tokyo, 3. Faculty of Env. Earth Sci., Hokkaido Univ.)
9:40 AM - 9:50 AM JST(12:40 AM - 12:50 AM UTC)Japanese

[[A]A301-4am-05]Identification of cyclic peptides that recognize post-translational modifications

○Sarino Kitayama1, Shin Watanabe2, Isao Suetake3, hironobu Hojo1 (1. Osaka university, 2. Tokuyama Corporation, 3. Nakamura gakuen university)
9:50 AM - 10:00 AM JST(12:50 AM - 1:00 AM UTC)Japanese

[[A]A301-4am-06]Synthetic study of selenocysteine-substituted nanobody

○Toshiki Takei1, Hironobu Hojo1 (1. Institute for Protein Research, Osaka University)
10:00 AM - 10:10 AM JST(1:00 AM - 1:10 AM UTC)Japanese

[[A]A301-4am-07]Solubilization of poorly soluble peptides by late-stage introduction of solubilizing tags to Asp/Glu side chains

○Moeno Sakurai1, Tetsuo Narumi1, Nobuyuki Mase1, Kohei Sato1 (1. Shizuoka Univ.)
10:10 AM - 10:20 AM JST(1:10 AM - 1:20 AM UTC)

Break

10:20 AM - 10:30 AM JST(1:20 AM - 1:30 AM UTC)Japanese

[[A]A301-4am-08]Computational Study on the Catalytic Mechanism of Prenyltransferase Fur7 in the Biosynthetic Pathway of Meroterpenoids.

○Takuto Ohmura1, Fan Zhao1, Yoshitaka Moriwaki1, Tomohiro Noguchi1, Taro Shiraishi1, Kuzuyama Tomohisa1, Tohru Terada1 (1. Dept. of Biotechnol., Grad. Sch. of Agri and Life Science., The Univ. of Tokyo.)
10:30 AM - 10:40 AM JST(1:30 AM - 1:40 AM UTC)Japanese

[[A]A301-4am-09]Theoretical study on difference in oxygen affinity when the cyclic π-conjugated systems are replaced in myoglobin

○Mai Harada1, Kohei Tada1, Ryohei Kishi1, Yasutaka Kitagawa1 (1. Osaka University)
10:40 AM - 10:50 AM JST(1:40 AM - 1:50 AM UTC)English

[[A]A301-4am-10]Fine tuning the substrate specificity of alcohol dehydrogenase from Geotrichum candidum by rational mutation

○Zhongyao TANG1, Guillermo Germán Otárola TEJADA1,2, Afifa Ayu KOESOEMA1, Tomoko MATSUDA1 (1. Institute of Science Tokyo, 2. University of Alcalá)
10:50 AM - 11:00 AM JST(1:50 AM - 2:00 AM UTC)Japanese

[[A]A301-4am-11]Secretory expression of albumin fused with green fluorescent protein using Pichia pastoris as a host

○Riko Higashi1, Mizuki Komori2, Nobutaka Fujieda1,2, Yoshitsugu Morita2 (1. Osaka Pref. Univ., 2. Osaka Metro. Univ.)
11:00 AM - 11:10 AM JST(2:00 AM - 2:10 AM UTC)Japanese

[[A]A301-4am-12]Construction of expression systems for Human serum albumin and its fusion proteins, and optimization of secretion signal sequence

○Mizuki Komori1, Nobutaka Fujieda1,2, Yoshitsugu Morita1,2 (1. Osaka Metro. Univ., 2. Osaka Pref. Univ.)
11:10 AM - 11:20 AM JST(2:10 AM - 2:20 AM UTC)Japanese

[[A]A301-4am-13]Effect of molecular crowding on dynamics of light-induced protein unfolding reactions

○Keigo Fujiwara1, Yusuke Nakasone1, Ikuya Nakaoka1, Honoka Ohta3, Soichiro Kawagoe2, Koichiro Ishimori3, Tomohide Saio2, Masahide Terazima1 (1. Kyoto Univ., 2. Tokushima University, 3. Hokkaido University)
11:20 AM - 11:30 AM JST(2:20 AM - 2:30 AM UTC)Japanese

[[A]A301-4am-14]Photoinduced oxidation reaction with malate dehydrogenase and zinc porphyrin linked viologen on ITO electrode

○Seiji Okamoto1, Hironao Ando1, Noriyuki Asakura1 (1. Institute of Science Tokyo)
11:30 AM - 11:40 AM JST(2:30 AM - 2:40 AM UTC)Japanese

[[A]A301-4am-15]Expression and purification of Cortisol oxidase (11 β-Hydroxysteroid Dehydrogenase 2) using E. coli.

○Hiroki Kaieda1, Kazuma Nohara1, Hiroya Hatano1, Takeshi Ikeda1, Takenori Ishida1, Ryuichi Hirota1, Akio Kuroda1, Hisakage Funabashi1 (1. Hiroshima Univ.)
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