[15a-K306-1~11] 3.3 Biomedical optics

11:30 AM - 11:45 AM JST(2:30 AM - 2:45 AM UTC)

[15a-K306-10] Development of a Single-Cell Secretion Imaging Technique Using Optical Waveguide-Based Total Internal Reflection Fluorescence Illumination

〇Zhuohao Yang¹, Takashi Kamatain², Nobutake Suzuki¹, Mai Yamagishi³, Takumi Adachi⁴, Koji Nagaoka⁵, Kazuhiro Kakimi⁵, Etsushi Kuroda⁴, Takashi Funatsu³, Shinya Sakuma⁶, Yasuyuki Ozeki¹, Yoshitaka Shirasaki¹ (1.The Univ. of Tokyo, 2.Science Tokyo, 3.Live Cell Diagnosis, Ltd., 4.Hyogo Med, 5.Kindai Univ., 6.Kyushu Univ.)

Keywords:

Fluorescence microscope,Cell imaging

In this study, we developed a technology that integrates wide-field total internal reflection fluorescence illumination using an optical waveguide chip with real-time fluorescence immunoassay, enabling high-throughput and single-cell-resolution imaging of cellular secretion. Furthermore, by applying deconvolution that accounts for the binding dynamics of antibodies to the acquired secretion images, we successfully reconstructed instantaneous secretion images for individual cells, allowing detailed visualization of spatiotemporal secretion dynamics.