[15a-P05-1~26] 12.7 Biomedical Engineering and Biochips

[15a-P05-6] Patterned culture of human iPS cell-derived cortical neurons using microfluidic devices

〇(DC)Mamoru Sakaibara^1,2, Hideaki Yamamoto^1,2,3, Kaneyasu Nishimura⁵, Sota Endo^1,2, Tomohiro Konno⁴, Shigeo Sato^1,2, Yoshito Masamizu⁵, Ayumi Hirano-Iwata^1,2,3 (1.Grad. Sch. Eng., Tohoku Univ., 2.RIEC, Tohoku Univ., 3.AIMR, Tohoku Univ., 4.Grad. Sch. Pharm. Sci., Tohoku Univ., 5.Grad. Sch. Brain Sci., Doshisha Univ.)

Keywords:

cultured neuronal network,microfluidic devices,iPS cell

In the field of neurological disease research, biomimetic systems using microfluidic devices and human induced pluripotent stem cell (iPSC)-derived neurons are becoming increasingly important. However, polydimethylsiloxane (PDMS), which is widely used in microfluidic devices, has the problem of adsorbing laminin-511, a scaffold substrate for human neurons. In this study, we inhibited the non-specific adsorption of laminin to PDMS using 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer and patterned cultured human iPSC-derived cerebral cortical nerve cells.