[17a-K403-1~9] 12.7 Biomedical Engineering and Biochips

9:15 AM - 9:30 AM JST(12:15 AM - 12:30 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[17a-K403-2] Functional verification of an insertion-type sucrose image sensor for visualization of photosynthetic products

〇Rintaro Okamoto¹, Yusuke Matsushita¹, Hideo Doi¹, Kotaro Takayama¹, Kazuaki Sawada¹, Kazuhiro Takahashi¹, Yong-Joon Choi¹, Toshihiko Noda¹ (1.Toyohashi Univ. of Tech.)

Keywords:

Puncture-type sensor,Agricultural Sensors,Sucrose Imaging in Plant Bodies

Our research group has successfully visualized pH changes and sucrose concentrations in plant stems using a plant insertion-type pH sensor. However, in the sucrose measurement, potential changes were detected using three enzymes and an electron mediator, but the enzymes were suspended in solution, making their application in plants difficult. In this study, the enzymes were immobilized on a potentiometric array using a photosensitive resin, and an insertion-type sensor was developed. The fabricated sensor indicated linear sensitivity in the range of 0.5 mM to 1 M (38.3 mV/dec). In an experiment using tomato side shoots, a sucrose solution containing ferrocene was added after the sensor was inserted, and the output potential of the sensor increased after about 1.4 hours, successfully visualizing sucrose in the plant. The observed potential response was 52.3 mV, which was calculated to be 1.1 mM in terms of concentration, demonstrating the accuracy of the sensor.