[17p-K201-1~7] Development of plant RI imaging technology and its application in agriculture

2:05 PM - 2:35 PM JST(5:05 AM - 5:35 AM UTC)

[17p-K201-3] Integrated analysis of the leaf sink-to-source transition using ¹⁴C-labelled photosynthate kinetics and gene expression analysis with RNA-seq data

Ai Kaiho-Soma¹, Ryohei Sugita², Yuko Kurita¹, Natsuko I. Kobayashi¹, Tomoko M. Nakanishi¹, 〇Keitaro Tanoi^1,3 (1.UTokyo, 2.Nagoya Univ., 3.F-REI)

Keywords:

photosynthate,gene expression analysis,sink-to-source

The balance between sink and source tissues is critical for plant growth and productivity. This study investigated the timing and mechanisms of the sink-to-source transition in soybean leaves. Using 14C-labeled carbon dioxide, we pinpointed the timing of the switch in photosynthate allocation during leaf development. RNA-seq analysis revealed significant changes in gene expression, including increased expression of genes involved in sucrose metabolism and transport, while sink-specific genes showed decreased expression. By integrating plant radioisotope imaging techniques with gene expression profiles, we explore the potential applications of these findings to enhance plant productivity.