[17p-K402-1~11] 12.6 Nanobiotechnology

3:00 PM - 3:15 PM JST(6:00 AM - 6:15 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[17p-K402-8] Effect of residual organic solvents on proton permeation through lipid bilayers

〇Yuto Yoshinare¹, Mana Honkawa¹, Yosuke Miki¹, Azusa Oshima², Masumi Yamaguchi², Akira Heya¹, Koji Sumitomo¹ (1.Univ. of Hyogo, 2.NTT Basic Res. Labs., BMC)

Keywords:

oil contamination,Giant Unilamellar Vesicles,ion penetration

Giant unilamellar vesicles, which are widely used as reaction fields for biomembrane mimicry, can be formed by various methods, including water-in-oil emulsion centrifugation method with organic solvents between lipid bilayers and electroformation method without organic solvents. In this study, we focused on protons, which have a higher membrane permeability coefficient than other ions and investigated the permeability of protons with and without organic solvents. The results showed that residual organic solvents significantly affected proton permeation and the membrane potential.