[1P01-32] Poster session

[1P31] Selective single-molecule detection of biomolecules
based on the electron transport properties of aptamer

*Ryohei Ishii¹, Tomoaki Nishino¹ (1. Science Tokyo (Japan))

A single-molecule junction, in which a molecule bridges nanogap electrodes, enables single-molecule identification based on electrical conductance. DNA aptamers exhibit superior molecular recognition for diverse targets, and combining aptamers with single-molecule measurements is expected to enable universal single-molecule detection. Using ATP as a model target, we fabricated a single-molecule junction of an ATP aptamer and investigated conductance changes upon complexation with ATP. In the absence of ATP, a single conductance state was observed, attributed to the aptamer junction alone. Upon ATP addition, an additional, higher conductance state appeared, assigned to the ATP-bound junction, reflecting changes in the aptamer's π-stacking structure. These results demonstrate that ATP binding alters the aptamer's conductance state, enabling single-molecule detection. Owing to the versatile recognition ability of aptamers, this approach can extend to diverse detection platforms.

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