[3D08-12] Soft Nanotechnology

2:45 PM - 3:00 PM JST(5:45 AM - 6:00 AM UTC)

[3D11] Comparison of accelerated activity of enzymes entrapped inside vesicles and liquid-liquid phase separation of enzymes

*Toshinori Shimanochi¹, Mizuki Nakatsuka¹, Yukitaka Kimura¹ (1. Okayama University (Japan))

Molecular crowding is caused by liquid-liquid phase separation, a phenomenon that separates two liquids by extreme differences in density, viscosity or solute concentration. This phenomenon is thought to be responsible for the creation of a highly efficient reaction field. Therefore, liquid-liquid phase separation is considered to be the key to explain the high activity of vesicle-encapsulated enzyme. In this study, to evaluate the liquid-liquid phase separation of model enzyme, carbonic anhydrase from bovine (CAB), we investigated the dissolution state of CAB in the inner aqueous phase of giant vesicles with a diamter of about 20 μm. As a result, CAB spontaneously underwent liquid-liquid phase separation, forming a highly concentrated and highly viscous region locally. It was suggested that the collision frequency with the substrate increased in the region, resulting in high enzymatic activity.

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