[3D08-12] Soft Nanotechnology

3:00 PM - 3:15 PM JST(6:00 AM - 6:15 AM UTC)

[3D12] Functional Analysis of Sodium Ion Channels and Development of a Drug Screening System Using a Device for Applying Membrane-Horizontal Voltage

*Koki Ohta^1,2, Tatsuya Nomoto^1,2, Maki Komiya², Yuzuru Tozawa³, Ayumi Hirano-Iwata^1,2,4 (1. Graduate School of Biomedical Engineering, Tohoku University (Japan), 2. Research Institute of Electrical Communication, Tohoku University (Japan), 3. Graduate School of Science and Engineering, Saitama University (Japan), 4. Advanced Institute for Materials Research (WPI-AIMR), Tohoku University) (Japan))

We developed an artificial cell membrane system applying a membrane-horizontal voltage (V_HORZ) to reactivate inactivated voltage-gated sodium channels. Since continuous measurement of these currents is difficult, we validated this system for drug screening using V_HORZ -evoked currents.The device features Au electrodes sputtered onto a micro-apertured Teflon film with CYTOP insulation. Cell-free synthesized sodium channels were reconstituted into artificial membranes formed within the aperture.Without V_HORZ, reconstituted channel openings were rare. However, applying V_HORZ significantly increased continuous channel activity. Adding tetrodotoxin (TTX) during V_HORZ application caused a clear concentration-dependent current reduction, confirming the activity originated from sodium channels. This demonstrates the V_HORZ platform's great potential for discovering novel ion channel modulators.

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