講演情報
[1B17]Efficient and cost-effective synthesis of N-acetyllactosamine via sequential modular enzymatic cascade reactions involving NTP regeneration
○焦 潤苗1、Li Jianjun2、Kamiya Kenji1、Qian Eika1 (1. Tokyo University of Agriculture and Technology、2. Institute of Process Engineering, Chinese Academy of Sciences)
キーワード：
Human milk oligosaccharides、multienzyme、biosynthesis
Human milk oligosaccharides (HMOs) play important roles in the brain development and health maintenance for infants, which are the third abundant components in the human milk. N-Acetyllactosamine (LacNAc) is an important intermediate for biosynthesis of other essential HMOs and antigens. Since the current synthetic methods including chemical and enzymatic ones are not appropriate for large-scale production of LacNAc, it is urgently needed to develop an efficient and cost-effective synthetic pathway of LacNAc. We established a one-pot multi-enzyme system to biosynthesize LacNAc and optimized the system. Finally, the LacNAc yield could be up to 90.5% under the low-cost condition, which would possibly meet the requirements of potential industrial production of LacNAc, and would provide a guide for production of other HMOs or functional oligosaccharides in the future.