Presentation Information
[1P47]Sensing of amyloidogenic proteins using gold nanoparticle-supported liposomes
*Tomohiro Sekiguchi1, Toshinori Shimanouchi1, Yukitaka Kimura1 (1. Graduate School of Environmental, Life, Natural Science and Technology, Okayama University)
Aggregation of amyloid beta peptide (Aβ) is a key factor in Alzheimer’s disease. The disease is considered to progress by the formation of rigid fibrils of Aβ via a template reaction. Liposomes, self-assembled bilayers made of amphiphilic molecules in water, are known to facilitate Aβ accumulation on their outer membranes. Also, gold nanoparticles (AuNPs), which exhibit localized surface plasmon resonance (LSPR), are often used in biosensors due to their modifiable surfaces and optical properties. In this study, we combined liposomes with AuNPs to prepare AuNPs-supported liposomes (AuNP@DPPC). Aβ(1-40), albumin, and lysozyme were absorbed onto the surface of AuNP@DPPC to alter LSPR from AuNPs. The absorbance of LSPR depended on Aβ(1-40) concentration even in the presence of albumin. This result suggests the possibility of an application of AuNP@DPPC to a biosensor.