Presentation Information
[1P51]Stochastic process in 2D-diffusion and association of Alzheimer’s disease-derived Aβ peptide on lipid planar membranes
*Toshinori Shimanochi1, Masaya Wakimoto1, Miki Iwamoto1, Yukitaka Kimura1 (1. Graduate School of Environment, Life, Natural Science and Technology, Okayama University)
Alzheimer’s disease is triggered by an aggregation of causative protein, amyloid β (Aβ). This process is likely to proceed on the neuronal cell membranes. Therefore, a deeper understanding of diffusion and association of Aβ would reveal a rigid mechanism of nucleation of Aβ that is a first step of Ab aggregation. In this study, a nucleation was visualized using fluorescently labeled Aβ and analyzed based on the autocorrelation function. Kinetic analysis of amyloid formation of Aβ on lipid membranes revealed a large dispersion of lag time, suggesting that the elongation rate is due to the nuclear structure. Further, a correlation function of Aβ estimated from its diffusion on lipid membranes indicated a contribution of Markov process. It is therefore suggested that the nucleation process of Aβ on lipid membranes is a stochastic process.