講演情報
[19p-B205-1][JSAP-Optica Joint Symposia 2023 Invited Talk] Ultrafast fiber lasers for multi-photon microscopy
〇Takao Fuji1, Hideji Murakoshi2 (1.TTI, 2.NIPS)
キーワード：
fiber laser、ultrashort pulse、multi-photon microscope
We introduce two femtosecond fiber laser systems for multiphoton microscopy, producing ~220 fs pulses at 1.3 μm and ~150 fs pulses at 1.8 µm, respectively. Starting from an erbium-doped silica fiber laser, we employ a highly nonlinear fiber for 1.3 μm conversion and a Raman shift fiber for 1.8 µm conversion. The pulses are amplified using Pr:ZBLAN and Tm:ZBLAN fiber amplifiers, reaching average powers of 0.1 W and 1.3 W, respectively. With the system of the Tm:ZBLAN fiber amplifier, we successfully image TurboFP635-expressing cortical neurons at a depth of 0.7 mm using three-photon excitation and Clover-expressing astrocytes at a depth of 0.15 mm using four-photon excitation with 1.8 μm pulses. Our findings demonstrate the potential of our laser system in advancing cellular imaging in deep tissues, enhancing our understanding of dynamic biological processes.