講演情報
[19p-B205-13]Microfluidic biochip of amorphous fluoropolymer CYTOP fabricated using two-photon polymerized 3D mold for high-resolution cell observation
〇Kotaro Obata1, Mirai Hanzawa1,2, Felix Sima1,3, Hiroyuki Kawano1,4, Kazunari Ozasa1, Yasutaka Hanada5, Godai Miyaji2, Atsushi Miyawaki1,4, Koji Sugioka1 (1.RIKEN RAP, 2.Tokyo Univ. of A. & T., 3.INFLPR, 4.RIKEN CBS, 5.Hirosaki Univ.)
キーワード：
CYTOP、Two-photon polymerization、Mold
Development of biochips which enables distortion-free imaging in the microchannel filled with a culture medium (water) is required to investigate behaviour of living cells in micro and nano environments. Fluoropolymer CYTOP (AGC Corporation) is a promising material with excellent properties such as high transparency in an ultraviolet region, high chemical resistance, and water repellence [1]. More importantly for the high-resolution observation of biological samples, its refractive index (1.34) is almost same as that of water (1.33), which is very attractive to be used as a substrate of biochip. However, its excellent material properties make it difficult to create 3D micro and nanostructures [2]. In this study, we have developed a new 3D fabrication method for CYTOP by using femtosecond laser based two-photon polymerized structures as molds.