講演情報
[20p-P09-6]Rapid detection of Pseudomonas aeruginosa exotoxin A by quantum dot fluorescent lateral flow immunochromatographic assay
〇(M1)Chiang YungChi1, He Chih-Hsuan1, Chang Yu-Han2, Fu Tsai-Sheng2, Lu Hsin-Chun1 (1.Chang Gung Univ., 2.Chang Gung Memorial Hospital)
キーワード：
quantum dot、fluorescence lateral flow immunochromatographic assay
This study investigated the detection of Pseudomonas aeruginosa (P. aeruginosa) exotoxin A (PEA) antigen by using quantum dot (QD) fluorescence lateral flow immunochromatographic assay (FLFICA) (QD-FLFICA), In order to provide rapid diagnosis of P. aeruginosa infection in clinical diagnosis, it is hoped that the time for bacterial detection can be reduced to less than 30 minutes without sacrificing the sensitivity and accuracy of the detection. The results of our study show that bioluminescent probes containing PEA detection antibodies and cadmium selenide (CdSe) QDs could be prepared from hydrophobic nano-sized CdSe QDs synthesized by microfluidic reaction through hydrophilic ligand exchange with 3-thiolpropionic acid (MPA), sequential surface modification with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS), and finally bioconjugation with PEA detection antibodies. QD-FLFICA for rapid detection of PEA antigens was developed by combining the above-mentioned bioluminescent probes containing PEA detection antibodies with FLFICA test strips containing immobilized capturing antibodies of PEA. The latest research results show that rapid detection of PEA antigens by the developed QD-FLFICA procedure could be achieved within 20 minutes by using the prepared bioluminescent QD probes containing PEA detection antibodies and the FLFICA test strips immobilized with PEA capturing antibodies. These results confirm the feasibility and potential of rapid detection of PEA antigens and, thus, P. aeruginosa infection by the developed QD-FLFICA procedure using the bioluminescent QD probes containing PEA detection antibodies and the FLFICA test strips prepared in this study.