講演情報
[20p-P10-5]Honeycomb Microchamber-Based Microfluidic Device for Single-Cell Analysis and Quantification of Cell-Secreted Biomolecules.
〇(P)Jonathan Briones1, Eiichi Tamiya1, Hyota Takamatsu2, Masato Saito1 (1.Osaka Univ., 2.Osaka Minami Med.Ctr)
キーワード：
microfluidics、functional assay、cytokine
We developed a microfluidic device with honeycomb-shaped microchambers for single-cell analysis and quantification of cell-secreted biomolecules. The device enables single-cell/multicellular systems, oil-droplet compartmentalization, and functional assays for cytokine detection at single-cell level. Standard lithography techniques were used for fabrication. Each microchamber is hexagonal shaped (h=20µm) surrounded by a 30µm flow channel. Fluid dynamics were simulated to optimize trapping capabilities, achieving up to 60% single-cell occupancy. Immune and reporter cells were paired in microchambers, allowing real-time monitoring and end-point analysis. GFP expression and SEAP secretion were used for cytokine detection. GFP expression was successfully observed in cell pairs over time, detecting specific cytokine-producing cells at the single-cell level. Cell staining and imaging techniques were employed for cell type identification. This device offers flexible and multifaceted single-cell analysis, providing insights into cell behaviors. Its applications include single-cell cytokine secretion monitoring, clinical diagnostics, and drug discovery.