講演情報
[21p-A302-10][INVITED] Air Plasma-activated Bio-medical Media for Cancer Therapy: Effective dose to LRI
〇Dheerawan Boonyawan1, Pipath Poramapijitwat1 (1.Chiang Mai Univ.)
キーワード：
bio-medical、atm air plasma、plasma-activated LRI
Atmospheric pressure (atm) cold plasmas could be utilized in various bio-medical applications due to their unique properties, such as their ability to directly generate reactive oxygen and nitrogen species (RONS). Nightingale® is an IEC-standard and CE-certified air-plasma device intended for medical use. For air-plasma-activated LRI (PA-LRI), pH changes, concentrations of H2O2, NO3-, and NO2-, as well as the stability, were investigated. Two-hour treatment of PA-LRI on A549 and H1299 cells resulted in morphological irregularity and cell death of 91.7 % and 70.0 %, respectively, while the non-cancerous cell line, human lung fibroblast (HLF), was not affected neither morphologically nor in terms of cell death. To elucidate the mechanisms of cell death induced by PA-LRI, common active species generated in PA-LRI were tested. Results showed that H2O2 alone can induce 72% of cell death, compared to PA-LRI, while 19% for NO2- and 2% for NO3-. The addition of catalase, which degrades H2O2, reduced cell death induced by PA-LRI and H2O2 to 9.2% and 6.4%, respectively. These results suggest that H2O2 is the most important player in PA-LRI-induced lung cancer cell death in vitro. To the best of our knowledge, this study is the first to investigate the shelf life of PA-LRI at room temperature for medical purposes, as well as the application of air-plasma activated-LRI on the in vitro inactivation of human lung cancer cells. Furthermore, cannabidiol-nanoemulsion (CBD-NE) was accumulated in the plasma-activated LRI to perform activity enhancement. The results will be presented for extensive discussion.