Presentation Information
[3P102]Electrochemical investigation of protein-protein interactions involving intrinsically disordered protein Praja1 on gold substrates
*Wataru Mori1,2, Takuya Nakanishi3, Wataru Onodera1, Terutoshi Kojima2, Toru Asahi1,3,4 (1. Graduate School of Advanced Science and Engineering, Waseda University, 2. Tsurugashima Development Center, OPTORUN Co. Ltd., 3. Comprehensive Research Organization, Waseda University, 4. Research Organization for Nano and Life Innovation, Waseda University)
Praja1 (PJA1) is an intrinsically disordered protein (IDP) and known to interact with aggregate-prone proteins such as alpha-synuclein (α-syn) in neurons. As IDPs are characterized by the lack of a fixed or ordered three-dimensional structure without their interaction partners, PJA1 is anticipated to retain its functionality in terms of protein interactions even on metal surfaces due to its flexibility, different from most structured proteins. In this work, we electrochemically investigated the behavior of PJA1 adsorbed on gold surface due to the presence of α-syn. It was indicated that the gold surface becomes exposed to solutions as a consequence of the conformational change in PJA1 by α-syn and thus the function of PJA1 remained even in its adsorbed state on gold substrate.