Presentation Information
[3P103]Direct delivery of target molecules into neurons by nanotube stamping
*Riko Kishida1, Riku Shibata1, Shota Yanagisawa1, Shun Fukabori1, Kazuhiro Oyama1, Yuiko Mizuguchi1, Takeo Miyake1, Takashi Tanii1 (1. Faculty of Science and Engineering, Waseda University)
The nanotube stamping is a technique in which numerous nanotubes are inserted into the cell membrane and substances are introduced directly into adherent cells through the nanotubes. The nanotube stamping can achieve both a high material introduction rate and a high survival rate. However, because this method involves inserting many nanotubes from above into cells adhering to a scaffold, it requires subcellular-level control of the position and tilt of the nanotube membrane. In particular, neurons require control at the subcellular level because of their weak adhesion. In this study, fluorescent substances were introduced into neurons by precise control of the relative positioning of the nanotube membrane to the cell/scaffold interface using a control system integrating microscope optics, acquisition image processing, and a stepping motor.