Presentation Information
[3P105]Preparation of artificial lipid bilayer membrane for molecular imaging of ion channels
*Juri Bando1, Haruka Okumura2, Yuzuru Tozawa2, Ryugo Tero1 (1. Toyohashi University of Technology, 2. Saitama University)
In this study, we aim to observe ion channel molecules by atomic force microscopy (AFM) using the supported lipid bilayer (SLB) system, which is an artificial cell membrane. Because the outer membrane region of ion channels has a size of approximately 1 - 3 nm, it is necessary to fabricate a smooth SLB with a surface roughness of less than a nanometer order, to observe the molecular images of the ion channels. Therefore, we aimed to optimize the sample preparation and AFM observation suitable for the observation of the ion channels. As a result, the roughness of the SLB surface observed by AFM was 0.69 nm (rms). This value is sufficiently smaller than the height of the outer membrane region of the ion channels (1 - 3 nm), and thus the asolectin SLB was suitable for their molecular imaging.