講演情報
[8p-P02-12]Large-scale data analysis of the stiffness of FUCCI HeLa cells grown on a ferroelectric crystal
〇ALEXIS BOROWIAK1,2, YOHEI KONO1,2, TAKESHI SHIMI1,2, TAKESHI FUKUMA1,2 (1.Kanazawa Univ., 2.NanoLSI)
キーワード：
Cell mechanics、Ferroelectric surface、Cell membrane
In the past decade, researchers have explored ferroelectric materials as virtual electrodes for studying biological specimens. Traditionally, applying a direct electric field to cells requires small, metallic electrodes, which may not be biologically compatible. Ferroelectric materials provide a promising alternative due to their spontaneous electric polarization, removing the need for an external circuit. This study examines the mechanical properties of FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) HeLa cancer cells grown on a ferroelectric crystal with different polarities using the JPK AFM QI mode, enabling the extraction of mechanical properties of cancer cell membranes taken in the G1 phase from 50,000 force curves. Our results demonstrate that ferroelectric surfaces can modulate the ionic environment of cells, affecting cell stiffness in response to the crystal's polarity and the surrounding ionic species.