講演情報
[15a-K305-4]金ナノ粒子結合による溶液表面における光捕捉誘起αシヌクレイン液滴形成の促進
〇柚 佳祐1,2、茶谷 絵理2、増原 宏1 (1.國立陽明交通大應用化學、2.神戸大院理)
キーワード：
光捕捉、タンパク質液滴、金ナノ粒子
Optical trapping, a technique to manipulate micro- and nanoparticles by mechanical force with a focused laser, has been developed in various research fields from optics to biology. Based on optical trapping, we have studied on optically evolved assembly of nanoparticles on solution surface, where nanoparticles assemble and grow beyond the laser focus area. We have previously discovered that lysozyme forms concentrated assembly in a supersaturated solution by optical trapping at solution surface, even though protein molecules are typically too small for optical trapping. In addition, we have recently found the optical trapping-induced formation of a single liquid-like condensate of α-synuclein at solution surface in its unsaturated condition. Here, we attempted a more effective α-synuclein condensate formation by conjugating gold nanoparticles (Au NPs), which have high polarizability due to surface plasmon resonance and are thus strongly optically trapped. In the presence of 10 nm Au NP-conjugated α-synuclein, condensate was formed with a lag time of approximately 30 sec by optical trapping. In contrast, without conjugation, a lag time of approximately 300 sec was required. Moreover, the condensate formed unstably and immediately deformed in the presence of free gold nanoparticles. These observations suggest that the conjugation of Au NPs is effectively promotes stable condensate formation.