講演情報

[15p-K306-3]Impacts of copper metal nanoparticle size on the internal activity of lentil (Lens culinaris) seeds using biospeckle optical coherence tomography (bOCT).

〇(D)Lavista Tyagi1, Hirofumi Kadono1, Uma Maheswari Rajagopalan2 (1.Graduate School of Science and Engineering, Saitama University,Japan, 2.Department of Mechanical Eng. Faculty of Engineering, Shibaura Institute of Technology, Japan)

キーワード:

Biospeckle Optical Coherence Tomography (bOCT)、Nanoparticles、Copper (Cu)

The increasing application of nanoparticles (NPs) in agriculture, medicine, and industry necessitates advanced methods for monitoring their effects on plants due to the uncertainties surrounding their impact on seed germination and growth. NPs can penetrate seed coats and interact with cellular processes, highlighting the need to understand their size-dependent effects. Our research employs Biospeckle optical coherence tomography (bOCT), developed in our laboratory, to observe the size-dependent effect of copper (Cu) metal NPs on the internal activity of lentil (Lens culinaris) seeds during germination. Understanding the impact of Cu NPs is crucial due to their widespread applications in electronics, catalysis, and biomedicine, as well as their unique properties like high electrical conductivity and significant catalytic activity. bOCT, a non-contact, non-destructive, in vivo monitoring technique, visualizes changes in the internal activity of biological objects. Our earlier study effectively confirmed its ability to assess the size-dependent effects of CuO NPs on lentil seeds and leaves, indicating a substantial negative impact of CuO NPs of <50 nm at 100 mg/L, while larger particles (<10 µm) showed positive effects at 25 mg/L. Additionally, leaf foliar exposure to CuO NPs at 100 mg/L resulted in significant negative effects within 24 hours.