講演情報
[17p-K406-2]Synthesis of Pyrene-doped Silica Nanoparticles and Their Application to Optical Oxygen Sensing and Singlet Oxygen Generation
〇(D)Noor E Ashrafi1, Nao Kojima1, Kouta Uchiyama1, Yasuharu Kanda1, Hideyuki Nakano1, Toshifumi Iimori1 (1.Muroran Inst. Tech.)
キーワード：
fluorescent nanoparticle、oxygen sensing、singlet oxygen
Dye-doped silica nanoparticles have garnered attention from researchers owing to their beneficial properties, such as signal amplification, surface modification, and biocompatibility. In this study, pyrene-doped silica nanoparticles (PSNPs) were synthesized through a water-in-oil microemulsion technique using pyrenebutyric acid conjugated with triethoxysilane to covalently entrap pyrene fluorophores within the silica matrix. The PSNPs were thoroughly characterized by TEM, FTIR, UV-Vis absorption, and fluorescence spectroscopy, which confirms the successful synthesis. The nanoparticles exhibited a spherical morphology with an average diameter of 72 nm and a fluorescence quantum yield of 27% for excimer emission. The impact of oxygen, acting as a collisional quencher, was examined, revealing a decrease in both fluorescence intensity and lifetime, indicating dynamic quenching. Ratiometric fluorescence sensing of oxygen concentration was demonstrated by analyzing the ratio between monomer and excimer fluorescence intensities. The biomolecular quenching rate constant was determined through the Stern–Volmer analysis of fluorescence lifetime data. Additionally, the use of a chemical trap for singlet oxygen molecules confirmed that singlet oxygen was produced via the photosensitization process of PSNP. The PSNPs synthesized in this study exhibit promising potential for applications in oxygen sensing, bioimaging, and a variety of environmental and industrial fields.