[2Cp01-10] 食品機能　抗酸化、その他食品機能

3:45 PM - 4:00 PM JST(6:45 AM - 7:00 AM UTC)

[2Cp04] Intervention of aggregates astaxanthin nano-delivery system on skin photoaging

*Yingyuan Zhao^1,3, Panpan Pei^1,4, Yuting Sun¹, Xiaole Feng², Xueqin Wang¹ (1. College of Biological Engineering, Henan University of Technology, 2. College of International Education, Henan University of Technology, 3. Laboratory of Nutritional Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, 4. Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences)

Keywords:

Aggregates astaxanthin,UVA/UVB,anti photo-aging

【Purpose】The skin is the most widely distributed and complex organ in the human body. Chronic skin damage caused by UVA/UVB-radiation, which is the main cause of skin photoaging in coastal areas. Astaxanthin, as the most powerful natural antioxidant ever discovered, demonstrates potential in mitigating UVA/UVB-induced skin-photoaging. Here, a novel astaxanthin nano-delivery system is proposed for alleviating skin photoaging【Methods】H/J-aggregates astaxanthin/BSA/Chitosan nanoparticles (H/J-ABC-NPs) were fabricated via a bottom-up layer-by-layer self-assembly technique. Oxidative damage was induced in L929 cells using H₂O₂ to verify the antioxidant effects of H/J-ABC-NPs. UVA/UVB irradiation-induced BALB/c mice photoaging model was constructed to explore the anti-skin photoaging ability of H/J-ABC-NPs.【Results】 H-ABC-NPs and J-ABC-NPs were both spheres with particle size around 231±9 nm and 368±5 nm, respectively. The dominant driving forces of H/J-ABC-NPs formation mainly included electrostatic, hydrophobic interactions and hydrogen bonding. H/J-ABC-NPs showed excellent cell safety and antioxidant activity in L929 cells of H2O2-induced oxidative damage, which inhibited production of ROS, promoted expression of SOD and CAT. Meanwhile, H/J-ABC-NPs showed anti-photo-aging potential in UVA/UVB irradiation-induced BALB/c mice photoaging model, which reduced wrinkles of the skin and collagen fiber fractur. In addition, H/J-ABC-NPs alleviated the inflammatory response, oxidative damage and degradation of collagen by promoting expression of SOD, CAT, GSH-Px, HYP and HA, inhibiting the overexpression of MDA, IL-1α, IL-6, IL-1β and MMP-1 in mice.