[P001-P057] Poster Presentation Awards / International Poster Award for Young Researcher

[P048] Inhalation of beta-caryophyllene has a protective effect against nicotine-induced vascular degeneration

*Mayo Higashihara¹, Tomoko Sumi¹, Tatsuya Moriyama^1,2, Nobuhiro Zaima^1,2 (1. Department of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, 2. Agricultural Technology and Innovation Research Institute, Kindai University)

Keywords:

β-Caryophyllene,aorta degeneration,aroma component

【Purpose】Vascular degeneration is caused by the degeneration of components of the vascular wall due to environmental factors. Smoking is a major risk factor. Smokers can be prevented by quitting smoking, but there is no preventive method for secondhand smoke. We focused on β-caryophyllene (BCP) included in spices. Oral administrated BCP is reported to exhibit bioactivities, such as anti-inflammatory. We reported that inhaled BCP can affect metabolites in the liver. These results indicate that inhaled BCP has biological activity. In the present study, we evaluated the effects of BCP inhalation on nicotine-induced vascular degeneration.【Methods】①ddy mice were on nicotine intake and BCP inhalation two weeks , dissection was performed and aortae were collected. Pathological analysis and vascular tensile rupture test were performed on the obtained samples. ②ddy mice were given nicotine intake for 2 weeks to induce vascular degeneration. After 2 weeks of nicotine ingestion and 3 times weekly BCP inhalation, dissection was performed, and aorta was collected. Pathological analysis was performed on the samples obtained.【Results】①Degeneration of elastic fibers and increase in MMP-2 caused by nicotine intake were suppressed by BCP inhalation. These were suppressed by BCP inhalation. Mechanical stiffening of aorta caused by nicotine was also suppressed by BCP inhalation. ②Degeneration of elastic fibers and increase in MMP-2 caused by nicotine intake were suppressed by BCP inhalation. Furthermore, an increase in CD163, M2 macrophage marker, was observed with BCP inhalation.【Discussion】The results suggest that BCP inhalation has a protective effect nicotine-induced vascular degeneration.