Presentation Information
[3Ap10]Preparation and characterization of nanoparticles for bioactive food-medicine homologous micromolecules
*Wenhui Qu1, Ruixi Yu1, Panpan Pei1,3, Yatong Xiang1, Yingyuan Zhao1,2 (1. College of Biological Engineering, Henan University of Technology, 2. Laboratory of Nutritional Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, 3. Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences)
Keywords:
Food-medicine homologous,Bioactive micromolecule,Nano-delivery System
【Purpose】
Food-medicine homologous bioactive micromolecules were nutritional and therapeutic functions, such as fucoxanthin, curcumin and paclitaxel. Their natural structure and biological activity given them significant potential. However, poor water solubility and chemical instability limited their application. Here, the construction of nanoparticles to enhance bioactive micromolecule application.
【Methods】
Bioactive micromolecules/whey protein/chitosan nanoparticles were fabricated via a bottom-up layer-by-layer self-assembly nano-technique. The hydrated particle sizes of the nanoparticles were analyzed by DLS, and their morphologies were characterized by TEM and SEM. The spectra of nanoparticles were determined by CD, FTIR and UV-Vis.
【Results】
The morphology of nanoparticles were approximately spherical by SEM and TEM, the average hydrated particle sizes of PBC-NPs, H-FBC-NPs, and H-LPC-NPs were 210 nm, 171 nm, and 173 nm, respectively, and the other nanoparticles in were all in the range of 170-260 nm by DLS. The spectroscopy indicated that chitosan and BSA formed hydrophobic microdomain by the intermolecular electrostatic interactions. The UV-vis showed M-monomers, spectrally blue-shifted H-aggregates and spectrally red-shifted J-aggregates. The encapsulation efficiency (EE) of these nanoparticles were all above 80%, and reaches up to 95%. The stability results showed that these nanoparticles were able to stabilize at 4-25°C environments for 48 h. In conclusion, the aqueous dispersion and stability of these micromolecules were significantly improved by constructing nanocarriers.
Food-medicine homologous bioactive micromolecules were nutritional and therapeutic functions, such as fucoxanthin, curcumin and paclitaxel. Their natural structure and biological activity given them significant potential. However, poor water solubility and chemical instability limited their application. Here, the construction of nanoparticles to enhance bioactive micromolecule application.
【Methods】
Bioactive micromolecules/whey protein/chitosan nanoparticles were fabricated via a bottom-up layer-by-layer self-assembly nano-technique. The hydrated particle sizes of the nanoparticles were analyzed by DLS, and their morphologies were characterized by TEM and SEM. The spectra of nanoparticles were determined by CD, FTIR and UV-Vis.
【Results】
The morphology of nanoparticles were approximately spherical by SEM and TEM, the average hydrated particle sizes of PBC-NPs, H-FBC-NPs, and H-LPC-NPs were 210 nm, 171 nm, and 173 nm, respectively, and the other nanoparticles in were all in the range of 170-260 nm by DLS. The spectroscopy indicated that chitosan and BSA formed hydrophobic microdomain by the intermolecular electrostatic interactions. The UV-vis showed M-monomers, spectrally blue-shifted H-aggregates and spectrally red-shifted J-aggregates. The encapsulation efficiency (EE) of these nanoparticles were all above 80%, and reaches up to 95%. The stability results showed that these nanoparticles were able to stabilize at 4-25°C environments for 48 h. In conclusion, the aqueous dispersion and stability of these micromolecules were significantly improved by constructing nanocarriers.
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