講演情報
[P154]薬用食品成分を標的とした低分子ナノデリバリーシステムの構築及びその特性評価
*Qu Wenhui1、Yu Ruixi1、Pei Panpan1,3、Xiang Yatong1、Zhao Yingyuan1,2 (1. 河南工業大学生物工程研究科、2. 名古屋大学大学院生命農学研究科栄養生化学研究室、3. 山東省農業科学院食品と栄養科学技術研究所)
キーワード：
食品および医薬品由来の低分子、小活性分子、ナノ・デリバリー・システム
【Purpose】
Food-medicine homologous bioactive micromolecules were nutritional and therapeutic functions, such as fucoxanthin, curcumin and paclitaxel. Their natural structure and biological activity given them significant potential. However, poor water solubility and chemical instability limited their application. Here, the construction of nanoparticles to enhance bioactive micromolecule application.
【Methods】
Bioactive micromolecules/whey protein/chitosan nanoparticles were fabricated via a bottom-up layer-by-layer self-assembly nano-technique. The hydrated particle sizes of the nanoparticles were analyzed by DLS, and their morphologies were characterized by TEM and SEM. The spectra of nanoparticles were determined by CD, FTIR and UV-Vis.
【Results】
The morphology of nanoparticles were approximately spherical by SEM and TEM, the average hydrated particle sizes of PBC-NPs, H-FBC-NPs, and H-LPC-NPs were 210 nm, 171 nm, and 173 nm, respectively, and the other nanoparticles in were all in the range of 170-260 nm by DLS. The spectroscopy indicated that chitosan and BSA formed hydrophobic microdomain by the intermolecular electrostatic interactions. The UV-vis showed M-monomers, spectrally blue-shifted H-aggregates and spectrally red-shifted J-aggregates. The encapsulation efficiency (EE) of these nanoparticles were all above 80%, and reaches up to 95%. The stability results showed that these nanoparticles were able to stabilize at 4-25°C environments for 48 h. In conclusion, the aqueous dispersion and stability of these micromolecules were significantly improved by constructing nanocarriers.
Food-medicine homologous bioactive micromolecules were nutritional and therapeutic functions, such as fucoxanthin, curcumin and paclitaxel. Their natural structure and biological activity given them significant potential. However, poor water solubility and chemical instability limited their application. Here, the construction of nanoparticles to enhance bioactive micromolecule application.
【Methods】
Bioactive micromolecules/whey protein/chitosan nanoparticles were fabricated via a bottom-up layer-by-layer self-assembly nano-technique. The hydrated particle sizes of the nanoparticles were analyzed by DLS, and their morphologies were characterized by TEM and SEM. The spectra of nanoparticles were determined by CD, FTIR and UV-Vis.
【Results】
The morphology of nanoparticles were approximately spherical by SEM and TEM, the average hydrated particle sizes of PBC-NPs, H-FBC-NPs, and H-LPC-NPs were 210 nm, 171 nm, and 173 nm, respectively, and the other nanoparticles in were all in the range of 170-260 nm by DLS. The spectroscopy indicated that chitosan and BSA formed hydrophobic microdomain by the intermolecular electrostatic interactions. The UV-vis showed M-monomers, spectrally blue-shifted H-aggregates and spectrally red-shifted J-aggregates. The encapsulation efficiency (EE) of these nanoparticles were all above 80%, and reaches up to 95%. The stability results showed that these nanoparticles were able to stabilize at 4-25°C environments for 48 h. In conclusion, the aqueous dispersion and stability of these micromolecules were significantly improved by constructing nanocarriers.
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